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Histology, immunohistochemistry and immunofluorescence staining for lung tissues Formalin-fixed and paraffin-embedded lung tissues of mice were cut in sections, which were stained with hematoxylin and eosin (HE), Periodic acidSchiff (PAS) and MASSON to respectively evaluate airway inflammation, goblet cell hyperplasia and mucous secretion, and extracellular matrix in the lung tissues [26]

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This system does not include growth restrictions as criteria, although clinicians are urged to assess and note any growth issues

Smoke-free products should produce less smell than cigarettes and not generate cigarette smoke smell

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