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Slides were then cooled to room temperature on ice and washed three times with TBST (1 TBS, 0.5% Triton X-100) before blocking in Block-B buffer (1 TBS, 3% BSA, and 0.1% Triton X-100) for 30 min at room temperature

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Certain areas of the body, such as the earlobes, shoulders, and chest, are more prone to these types of scars, due to the higher concentration of collagen-producing cells in these regions