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Flow rate was set to 100 L/min for 5 min with 0% mobile phase B, then, switched to 400 L/min for 50 min, with a linear gradient of mobile phase B from 20% to 100%

As it will be presented later, one of the critiques to the use of fear is that it is working for short term period

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HEPA : This type of air filter is rated to use mechanical filtration to capture 99.97% of particles 0.3 microns in size, which includes some smoke particles