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Neither loss nor gain of LAS1 function resulted in developmental defects or alteration in sterol profiles in Arabidopsis, suggesting cycloartenol as the primary and predominant pathway over lanosterol (Suzuki et al., 2006)

Vectors encoding RfA-cassette with or without N-terminal epitope tag(s) The RfA-cassette encoding a chloramphenicol resistance marker and ccdB flanked by attR1 and attR2 was amplified by PCR using primers Dest-5-SnaMfePac and Dest-3-SphPspAvr