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Ideally, keep them in one layer but if your container can't hold them that way, separate the layers with non-stick baking paper

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3.9 Analysis of the transcriptomic profiles and correlations between the transcriptome and proteome of tobacco seedlings in response to chilling stress The differential transcriptional regulation of tobacco seedlings in response to chilling stress was studied by analyzing the transcriptomes of the shoot and root after 24 h of chilling treatment

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