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Loss of epithelial barrier integrity is a hallmark of airway injury and occurs in airway diseases including COPD, acute respiratory distress syndrome, asthma, fibrosis, and EMT/cancer, thus supporting the conclusion that the application of liquid to dpHBEC causes a phenotype that is consistent with a range of respiratory diseases (Figure 5) is not sufficient to prevent the loss of barrier integrity following liquid application

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Many times, these lifestyle factors are familial or related to childhood living conditions