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Knowing When to Let It Go (or Fire It Back Up) Theres no exact rule for when to put down a cigar, but most folks stop when the flavor turns or when the heat creeps too close to the fingers

All samples are decanted and measured using sterile tools

Thus, to further test the polarity of the later ciliating and mature ciliated expression states in the ALI cultured scRNA-seq dataset, we used the Velocyto pipeline 78 (applying default options) to identify unspliced and spliced mRNA reads for each gene in the original in vitro BAM files