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Complete blockade of this pathway may provide effective relief from itch but could potentially disrupt immune homeostasis, raising concerns about immune rebound and proinflammatory flare-ups ( MRGPRX2, a receptor selectively expressed in primates, presents further challenges due to the absence of a natural ortholog in rodents, which complicates the development of translational animal models

Bacteria were pelleted in microcentrifuge tubes at 13000 g for 10 min and washed twice in PBS