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The same process was repeated for subsequent antibodies and fluorescent dyes: anti-Scgb1a1/TSA 570 (1:200, MA5-29780, Thermo Fisher Scientific, Waltham, MA, USA), anti-Lsm2/TSA 620 (1:200, NBP2-38093, Novus, Littleton, CO, USA), and anti-Krt5/TSA700 (1:100, ab64081, Abcam, Cambridge, UK)

Steady moderate smokers appeared to be the most common group, accounting for 29.52% of the participants, followed by never smokers (22.05%), decreasing light smokers (20.22%), and new current smokers (12.75%)

Whether you require employees to ID everyone, or people who look under 35, or whatever make your policy crystal clear

I didnt think about the impact it was having on them

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