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The PCR products were separated on a 1.0% agarose gel, and purified using the QIAEX II gel extraction kit (Qiagen, Valencia, CA)

While these arent always 100% failproof, they can help prevent the alarm from triggering for no reason

The pupal stage, which occurs in the soil or substrate, is key to understanding why traditional chemical control fails: if you only treat the aerial part of the plant, the pupae in the substrate will survive and give rise to new adults

The youth leaders made presentations to local community groups to raise awareness of the state of retail and healthy-food access in the Tenderloin

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