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Sections were pre-incubated in 5-guanylate diphosphate (2 mM GDP) for 15 min at room temperature, and then incubated for 60 min in HEPES buffer containing GDP (2 mM) and [ 35 S]GTPS (40 pM), with or without 8-OH-DPAT (1 M)

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Further, the rule now specifies that a representative of the 13CT licensee must be present at all times during the catered contracted event, which targets the illegal renting of a license by ensuring that the person responsible for the sale of alcoholic beverages is in fact associated with the identified licensee rather than a third-party