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A reversed phase phenyl-hexyl column (Luna Phenyl-Hexyl, 3 m, 150 2 mm, Phenomenex, Aschaffenburg, Germany) with a gradient system consisting of 0.1 % formic acid (A) and 0.1 % formic acid in acetonitrile (B) was used to separate out the nicotine

In order to establish emphysema model, a volume of 0.3 mL CSE solution was intraperitoneally injected to mice on days 0, 11, and 22, while 0.3 mL PBS were intraperitoneally injected to mice in the control group on days 0, 11, and 22