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Utilizing isolated components like nicotine or cigarette smoke extract (CSE), it has been discovered that CS inhibits NO production in vitro (21, 39)

Mitochondrial function and mitochondrial DNA (mtDNA) quality were shown to decline with age in postmitotic tissues 14,15,16,17 , and several recent studies, reporting on defects in mtDNA quality control mechanisms in mice, have shown that these mutant animals develop a phenotype of premature aging 17,18

The cumulative exposure to chemicals and particulates over years of service can result in respiratory conditions, heart disease or cancer

and significantly increased measures of arterial stiffness, a key risk factor in the development of cardiovascular conditions such as heart attack or stroke

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