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The core laryngeal microbiota was dominated by Comamonadaceae , and increased Streptococcus abundance in benign vocal fold disease suggested its potential role in disease development

Indeed, many of the observed neurovascular and metabolic effects of both cigarettes and e-cigarettes was reported to attributed to nicotines action on nicotinic acetylcholine receptors (nAChRs) such as nicotine alone can increase BBB permeability via endothelial nAChR signaling and alter cerebral glucose utilization [30, 31]

Meanwhile, nicotine toxicity may be mediated through changes in collagen fibers that will prevent proper sperm release from germinal epithelium into middle cavity of spermicidal tubes [37, 38]

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