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Analytes were separated using a gradient elution at 0.4 mL/min under the following conditions: 0.5 min with 90% buffer A (5 mM ammonium acetate) and 10% buffer B (100% MeOH), followed by a linear gradient for 3.0 min to 70% buffer B and a subsequent linear gradient for 1.0 min to 90% buffer B

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