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Materials and Methods Introduction of the Metal Binding and Reducing Peptide Sequence into C-terminal Surface Exposed Loops of TMV Coat Protein Plasmid pSNC004 containing a full-length TMV U1 genome under the control of a T7 promoter and modified in its coat protein sequence at nucleotides 193198 and 208213 to contain NgoMIV and BstZ17I sites, respectively, was provided by Dr Sean Chapman (The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK)

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By being closely located to its clientele, therefore sparing people the long travel to remote warehouses, the supermarket chain allowed its customers to shop for themselves, coining the new "cash and carry" concept