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Passage 2 cells were treated with MitoQ (50 nM, HY-100116, MCE), fenofibrate (10 M, T1149, Topscience), oleic acid (10 M, S4707, Selleck), etomoxir (50 M, S8244, Selleck), bafilomycin (5 nM, S1413, Selleck) and SRT1720 (4 M, S1129, Selleck) (Additional file 1)