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Briefly, cells were lysed on ice with ice-cold 1% Brij-96 containing lysis buffer (50 mM Tris-HCl, pH 7.2, 150 mM NaCl, 1 mM orthovanadate, 1 mM phenylmethylsulfonyl fluoride, 10 g/ml aprotinin, and 10 g/ml leupeptin) for 15 min

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Similar content being viewed by others Log in or create a free account to read this content Gain free access to this article, as well as selected content from this journal and more on nature.com or References Abreu ME, Bigelow GE, Fleisher L (2001)

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