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Chlorophyll a (Chla), chlorophyll b (Chlb), and carotenoid (Car) contents were determined by measuring absorbance at 665, 649, and 470 nm, respectively ( 2.2.4 Tobacco leaf glandular trichomes and their secretion content At the vigorous growth and maturity stages, fresh middle leaves (9th position from the top) from three randomly selected plants per treatment, avoiding the main veins, were cut and divided into two samples

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