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Nicotine binds to and activates the nicotinic acetylcholine receptors (present abundantly in the central and peripheral nervous systems) and thus increases intracellular calcium and leads to downstream activation of cAMP response element-binding protein, a key transcription factor for many genes (Shen and Yakel, 2009)

American Spirit cigarettes typically contain more tobacco filler, resulting in more puffs per cigarette and increased exposure to tobacco chemicals

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Citation2012), tobacco lines, and producing areas (Shi et al

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