sgOCT4 CRISPRa Controls Vector | Format – SELECT ONE::sgOCT4 Dual sgRNA in pRSL10Ble-mU6-sg1-hU6-sg2-UbiC-Tag RFP-2A-Bleo | Plasmid Lentivirus expressing luc with BlastR

Lentivirus expressing luc with BlastR (virus)

BFP) as well as a second construct with a non-target sgRNA and a second fluorescent marker (usually RFP or BFP)

Cellecta’s K562 cell lines are engineered for use as antigen-presenting cells (APCs) in T-cell receptor (TCR) functional assays

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Manual and Product Documentation Manual: CRISPR Pooled Lentiviral sgRNA Libraries

sgOCT4 CRISPRa Controls Vector | Format<span class="variant-dropdown"> – SELECT ONE:</span>:sgOCT4 Dual sgRNA in pRSL10Ble-mU6-sg1-hU6-sg2-UbiC-Tag RFP-2A-Bleo | Plasmid Lentivirus expressing luc with BlastRCellecta's sgOCT4 CRISPRa control constructs are human OCT4targeting sgRNAs in Cellectas standard CRISPRa lentiviral backbones, available either as plasmid DNA or packaged lentiviral particles for direct transduction. SinglesgRNA controls in pRSG16 U6 (sg) UbiC RFP Puro provide a straightforward positive control for CRISPRa activation, while the dualsgRNA pRSL10Ble mU6 sg1 hU6 sg2 UbiC TagRFP 2A Bleo formats offer stronger promoter activation and