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After successfully knockdown of Sat1 mRNA and protein expression in H9C2 (Figures 4AC), it was observed that the inhibition of Sat1 significantly rescued cell viability (Figure 4D) and decreased LDH release after OGD/R (Figure 4E)

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Therefore, GST as an affinity tag becomes a versatile tool for single-step purification of active recombinant production in a prokaryotic expression system

coenzyme A (CoA) is universally involved in metabolism of lipids, amino acids, and carbohydrates

Bioinformatics and biochemical studies show that Ptzyme@D-ZIF prevents neuroinflammation-induced apoptosis and ferroptosis (120) in damaged neurons