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qPCR confirmed that the effective downstream cytokines/growth factors (VEGFa, PDGF, FGF, TNF, IGF-1 and BDNF) of these pathways are upregulated by erucamide, but they were dysregulated when we knocked down TMEM19 (Fig

Cells were initially pre-cultured in 5 ml of YPDA or SCDropout medium (adjusted to meet auxotrophic requirements for plasmid maintenance) in a cell culture tube rotating overnight at 30 C