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Nat Immunol 18:12881298 Hansen RJ, Balthasar JP (2002) Effects of intravenous immunoglobulin on platelet count and antiplatelet antibody disposition in a rat model of immune thrombocytopenia

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In practice, combine species reporters that resolve flux and locatione.g., DHE-HPLC to quantify O 2 specifically via 2-OH-E (not raw fluorescence), compartment-targeted HyPer variants to track H 2 O 2 in the matrix/IMS/ER/nucleus and NO/ONOO probes applied with kinetic discrimination and appropriate scavenger/enzyme controlswith footprints of damage that integrate exposure over time (3-nitrotyrosine for nitrative stress, lipid peroxides/F 2 -isoprostanes or MDA/HNE adducts for membrane injury and protein carbonyls for irreversible protein oxidation) (Murphy et al

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