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fabG emerges as a key enzyme, catalyzing the initial reduction reaction in fatty acid synthesis, mostly using NADPH as a coenzyme to facilitate the conversion of 3-ketolipid acyl ACP to 3-hydroxylipid acyl ACP

The average recoveries for spiked sample ranged from 81.22% to 113.67%, with relative standard deviations between 3.22% and 12.55%, indicating high sensitivity, accuracy, and precision of the method

However, this approach faces several challenges, including the potential for the blood-brain barrier (BBB) to mask or dilute CNS-specific signals and the difficulty in distinguishing brain-derived markers from those originating systemically (87,88)

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( D ) Relative expression of CHAC1 in liver biopsies from MASLD/MASH