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Denaturing conditions (8 M guanidineHCl, pH 1.5 or boiling in SDS-sample loading buffer) are required to efficiently dissociate streptavidin -biotin complexes

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Reagent information Human HaCaT keratinocytes (Cell Lines Service GmbH, Eppelheim, Germany) were maintained in Dulbecco's Modified Eagle Medium/high glucose (Hyclone Laboratories, South Logan, UT, USA) containing 10% fetal bovine serum (Gibco, Life Technologies Co., Grand Island, NY, USA) and an antibiotic-antimycotic (Gibco) at 5% CO 2 , 37 C, and humidified atmosphere conditions