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The expression of the shRNA constructs was induced by incubating cells with 2 g ml 1 doxycyclin

Recently, newer DAAs (simeprevir and sofosbuvir) in combination with PEG-IFN and RBV have become available for genotype 1 patients.[76, 77, 99, 100] The advent of protease inhibitors has improved the likelihood of cure, but with a number of inherent limitations: (i) they do not have anti-viral activity in HCV genotypes other than HCV-1