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Arthritis Rheum (2000) 43(11):250112

To minimize non-specific binding, the membrane was then blocked with 5% bovine serum albumin (BSA) for a duration of 1 h at ambient temperature

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The resulting extract was then centrifuged for 15 min at 20,000 g

For him 'the whole picture fell into shape' once he began to focus on the idea of antibodies as cell receptors and eliminated the role of circulating antibodies in the early stages of antibody formation