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Classification and functional annotation of metabolites were performed based on the Human Metabolome Database (HMDB, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database ( Partial least squares discrimination analysis (PLS-DA) was employed to identify differentially abundant metabolites between groups, utilizing the opls function with parameters orthoI=0 from the ropls R package (v1.34)

Menzel T., Rahman Z., Calleja E., et al., Elevated Intracellular Level of Basic Fibroblast Growth Factor Correlates With Stage of Chronic Lymphocytic Leukemia and Is Associated With Resistance to Fludarabine, Blood 87 (1996): 10561063

We also predict that urea, cleaved from arginine, can be hydrolysed into two carbon dioxide molecules and ammonia

With the recent broad recognition of click chemistry and bioorthogonal chemistry, we anticipate a surge in research in the years to come, driving further advancements in biocompatible techniques for peptide macrocyclisation and expanding the toolkit of chemical methods for protein and cellular ligation