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The percent (%) cytotoxicity was determined using the following formula for each given experimental treatment: Cytotoxicity (%) = 100 [(Experimental Culture Medium Background)/(Maximum LDH Release Culture Medium Background)]

When the retina is involved, in case of retinal blood vessels, it is called retinal vasculitis, vitritis (vitreous), and when the optic nerve is involved, it is papillitis (Char and Schlaegel 1982)

For better understanding, several studies have focused on the anticholinesterase role of Piper nigrum extracts and constituents in animals

Gel permeation chromatography A Superdex 200 HR 10/30 column run on an kta purifier system (GE Healthcare GmbH, Freiburg, Germany) was equilibrated under anaerobic condition in an anaerobic chamber with protein buffer I and calibrated using the Gel Filtration Molecular Weight Markers Kit MWGF200 (Sigma Aldrich, Chemie, Taufkirchen, Germany) composed of cytochrome c ( M r = 12,400), carbonic anhydrase ( M r = 29,000), bovine serum albumin ( M r = 66,000), alcohol dehydrogenase ( M r = 150,000), -amylase ( M r = 200,000), and apoferritin ( M r = 400,000) according to the manufacturers instructions

Baizabal Carvallo, J