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Importantly, iodoacetamide (IAA, a known thiol-reactive compound) was not able to oxidize roGFP when cells were exposed to non-lethal or subtoxic concentrations of this compound, indicating that an electrophilic attack on roGFP is not sufficient to cause a spectral change consistent with its oxidation (Supplementary Fig

2.1 Animal-derived alternatives Animal-derived alternatives mainly include animal sera, platelet lysates and tissue extracts (Wijerathna-Yapa et al., 2025), supplying essential growth factors, hormones and proteins for cell proliferation and survival, and are more cost-effective than FBS

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