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Immunoblotting To profile protein lipoylation and lysine glutarylation, 0.5 10 6 HeLa cells or 1 10 6 T cells were lysed in SDS loading buffer (2% (wt/vol) SDS, 50 mM Tris (pH 7.4), 150 mM NaCl, 1 mM dithiothreitol, 10% (vol/vol) glycerol and 0.2% (vol/vol) benzonase nuclease (Sigma-Aldrich, E1014)) and heated at 90 C for 5 min

We measured peripheral neurotrophin concentrations via an enzyme-linked immunosorbent assay

References International Journal of Molecular Sciences (MDPI) Elamipretide: comprehensive review of structure, mechanism of action, and therapeutic potential British Journal of Pharmacology (PMC) Cardiolipinbinding mechanism and mitochondrial membrane stabilization by SS31 U.S

This is used to treat male hypogonadism in individuals who have failed natural therapy options