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NetID annotated 7,015 peaks as artefactual (including isotopes, adducts, fragments, and ringing artifacts), 2,369 peaks as known metabolites, 2,305 peaks as putative derivatives of known metabolites, and 2,685 peaks as putative unknowns

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Notably, several genes involved in glycolysis/gluconeogenesis (GNG) pathways, such as alcohol dehydrogenase 7 ( Adh7 ) and glucokinase ( Gck ), and genes related to antioxidant defense, including gamma-glutamyltransferase 6 ( Ggt6 ), glutathione S-transferase pi 3 ( Gstp3 ), and microsomal glutathione S-transferase 2 ( Mgst2 ), were downregulated in response to SIRT1 deletion (Fig

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Animal studies have shown that when beta-3 adrenergic receptors were genetically removed, chronic AOD-9604 treatment produced no changes in weight or lipolysis, confirming the importance of this pathway