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The 10 l of enzymatic extract was mixed with 90 l of solution consisting of 5 mM guaiacol and 2.5 mM H 2 O 2 in 50 mM acetic buffer (pH 5.6.) Activity measurement was assayed at 37 C in a Nunc U-bottom 96-well plate on a Varioskan LUX Multimode Microplate Reader

Primarily synthesized in the liver, glutathione performs several key functions: Neutralizes free radicals that cause oxidative stress and cellular damage

Extracellular cysteine is relatively unstable and readily autoxidizes to cystine, which can lead to the production of toxic oxygen free radicals [83,87,91]

Herpesviruses are extremely efficient at establishing chronic infections 8 and may cause severe disease in both domestic and free-ranging animals 9,10

Pound, N., Javed, M