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In brief, 50-100 mg leaf samples were frozen in liquid nitrogen, and ground with 1 mL Extraction Buffer [50 mM EPPS buffer, pH 8.0, containing 1 mM EDTA, 10 mM DTT, 0.1% Triton X-100 [v/v], 0.5% polyvinylpyrrolidone, and 1x SigmaFAST Protease Inhibitor Cocktail, EDTA Free (Sigma)] on ice using 2 mL glass-to-glass homogenizer

Zinc (10 mg): Critical for wound healing, immune system modulation, and skin health

We have summarized existing and emerging treatment protocols in Table 3 to highlight the characteristics and application scenarios of different treatment strategies

It is the final step of protein metabolism

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