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Sections were then incubated with primary antibodies against human collagen type II and human 2 microglobulin (Abcam, Cambridge, UK) diluted in 4% bovine serum albumin for 1 h at 37 C

During this process, myocardial cells undergo pathophysiological changes that cause notable damage

Severe hemorrhage is associated with high NO levels.67,68 Thus, V-B12 may be a viable, temporary resuscitation treatment option when blood is unavailable, but it has not been tested in a trial.67 The increase in NO in hemorrhagic shock (and septic shock) results from upregulation of NOS by inflammatory cytokines, as well as the release of H 2 S.66 Bebarta et al

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It is also needed for the heart, muscles and nerves to function properly