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Sequencing was performed on the Illumina platform Novaseq X Plus at the Northwestern University NUSeq sequencing facility core, with a targeted sequencing depth of 20,000 and 25,000 reads per sample acquired for RNA and ATAC profiling, respectively

To evaluate whether mutants deficient in different glutathione-dependent enzymes are impacted by TMHT, we subjected cytosolic glutaredoxin mutants to TMHT (Figure 4C)

Differential expression analysis was performed in R studio (R version 3.6.3) using the DESeq2 package with a q-value 1.5

In diabetic patients, metabolic abnormalities can lead to alterations in amino acid signaling pathways, which, in turn, affect mTORC1 activity and disrupt the autophagy process (Singh et al., 2024)

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