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At 16 h post-transfection, cells were collected, cryogenically snap-frozen into aliquots to ensure consistent performance across screens and seeded into 1536-well plates pre-spotted with test compounds at 4050% cell confluency in 5 L

After digestion, 300 L of 50% ACN/5% formic acid (FA) solution was added to the tubes, which were incubated in a water bath with sonication for 1020 min

Hepatic stellate cells support hematopoiesis and are liver-resident mesenchymal stem cells

Samples of 18 captive plains zebras were collected from the Rserve Africaine de Sigean (5 females and 4 males), Thoiry zoo (4 males) and Givskud zoo (2 females and 3 males), respectively

Le mcanisme identifi tait la perturbation de l'axe ERO-p53 : en rduisant le stress oxydatif intracellulaire, les antioxydants diminuaient l'expression de la protine p53, un suppresseur de tumeur majeur dont l'activation dpend prcisment de la prsence d'ERO