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Thermo Fisher Scientific) for 1 h at 4C to absorb bead-binding nonspecific proteins and immunoprecipitated using 50 l of RFP-Trap Magnetic Agarose (rtma, ChromoTek) for another 1 h at 4C

FIGURE 4 Immunohistochemical expression of SIRT-1 in the hepatic tissue among different experimental groups Both the control and PA groups showed positive SIRT-1 expression, but the APAP group showed weak positive SIRT-1 expression

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Raynaud's syndrome Symptoms: The consequence is the paling of fingers on the hands or feet (sometimes it can be the nose or auricles)

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