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The cells were left to differentiate into MDMs for 7 days, then washed with PBS, treated with 5 mM PBS/EDTA at 37C for 20 min, harvested gently with a cell scraper, counted and replated on 96- or 6-well trays at 1104 and 1106 cells per well, respectively, as described previously (31)

The diversity and ubiquity of natural antioxidants in plants and animals are factors that elicit the continual discovery of their roles in these diseases

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doi:10.1016/s0029-7844(99)00610-9 [18] Gilfix BM

The added targets generally correlate with larger weight reductions in trials