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Tian Y, Li G, Zhang S, Zeng T, Chen L, Tao Z, et al

Loss of IL-1 K133 ubiquitylation does not impede IL-1 activation or release To explore if IL-1 K133 ubiquitylation and/or the K133:D129 electrostatic interaction also impacted Caspase-1-mediated cleavage and release of IL-1, we co-transfected 293T cells, which lack GSDMD expression 27 , with Caspase-1 and Il1b cDNAs