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Our results showed that exposure to CdCl 2 increased cell death in the head region of zebrafish at 72 h post-fertilization (hpf) in a dose-dependent manner, with a significant elevation in the levels of reactive oxygen species (ROS) at concentrations of 1 M at 48 hpf

But there is actually an optional fourth step that you can do after youve collected your protein off of the column: washing and regenerating the beads so that you can use them again

Chiu CC, Keeling CI, Bohlmann J

In contrast, Gln deprivation abrogates c-Myc-mediated transcriptional repression, resulting in a significant upregulation of miR-29b-3p expression

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