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After centrifugation at 17,300 g at 4 C, the supernatant (350 g of protein) was incubated with 5.5 g of rabbit anti-HA antibody (Sigma-Aldrich

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5.1 Glycolytic fueling and intercellular transmission of metabolic reprogramming In the early stages of sepsis-induced ARDS, AMs undergo drastic metabolic reprogramming, rapidly shifting from using oxidative phosphorylation for energy production to aerobic glycolysisthe Warburg effectto meet the energy and biosynthetic demands for the rapid synthesis of inflammatory mediators (71)