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Tissues are then cocultured on a filter floating above serumfree media for 7 days

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Endotoxins may introduce unwanted variables into sensitive laboratory experiments

Ideal users: Patients requiring 50-100 units per injection Mixed insulin regimens (combining two insulin types in one syringe) Type 2 diabetes patients with significant insulin resistance Patients who prefer using one syringe rather than splitting doses Real-world example: Consider someone taking 70 units of basal insulin daily