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These cells were maintained in either RPMI-1640 or DMEM (Gibco, Massachusetts, USA), supplemented with 10% fetal bovine serum (Gibco), and incubated at 37 C in a humidified atmosphere containing 5% CO 2

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153 In addition, many firms, including Dicerna Pharmaceuticals (Dicerna), 156 Silence Therapeutics, 157 Sirna Therapeutics, 158 Nitto Denko Corporation, 159 Life Technologies 160 and Suzhou Ribo Life Science, 49,142 have explored proprietary delivery technologies for preclinical and clinical investigations

133 YangY.GehrkeS.ImaiY.HuangZ.OuyangY.WangJ.-W.et al (2006)

2023), and also used as a substrate of glutamine synthetase to detoxify ammonia and produce glutamine (Schousboe et al