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Anti-AGO2 and anti-IgG were used for immunoprecipitation, and qRT-PCR was used to analyze target RNA

Seguin A, Bayot A, Dancis A, Rogowska-Wrzesinska A, Auchre F, Camadro JM, Bulteau AL, Lesuisse E: Overexpression of the yeast frataxin homolog (Yfh1): contrasting effects on iron-sulfur cluster assembly, heme synthesis and resistance to oxidative stress

10.1186/s12967-018-1756-4 J Transl Med

For treatments, it would be beneficial to have a cellular source that can replicate in vitro to generate large numbers of cells and can then be coaxed into generating specific cell types, without forming tumors, in vivo