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The application of anti-cholinergic agents (scopolamine, atropine, 10 mg/kg b.w., i.p.) led to a significant cognitive deficit in the rats, a finding successfully reproduced assessing the animals behavior in water T-maze for a prolonged period

The apoptotic process involves distinct pathways with different protein, and can be triggered either by intrinsic factors, such as extensive DNA damage, ischemia, oxidative stress, or infections, or by extrinsic factors, which involve the interaction of specific membrane receptors with pro-apoptotic molecules produced elsewhere

Furthermore, inactivation of key androgenic enzymes, such as 3-hydroxysteroid dehydrogenase and 17-hydroxysteroid dehydrogenase, together with decreased testosterone synthesis suggest a potential impairment of male gonadal function

Kingsolver et al., 2015

Results can vary depending on the individual, but improvements are typically noticeable within a week after the first session