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The NPCs were cultured in poly-D-lysine (PDL, 10 g/ml

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Among individuals with CSF and plasma data, AD cases corresponded to those with a diagnosis of dementia of the Alzheimers type (DAT) using criteria equivalent to the National Institute of Neurological and Communication Disorders and Stroke-Alzheimers Disease and Related Disorders Association for probable AD 58 , and AD severity was determined using the Clinical Dementia Rating (CDR) 59 at the time of lumbar puncture (for CSF samples) or blood draw (for plasma samples)

CC analysis demonstrated that the differential proteins were mainly found in the extracellular region, cell junctions, and synapses, which indicated the reliability of the sample source (Fig