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C/EBPalpha arrests cell proliferation through direct inhibition of Cdk2 and Cdk4

Interestingly, mechanotransduction in aged individuals and those with glaucoma appear to be compromised across all tissues of the eye (cornea, sclera, TM, retina), suggesting broad dysfunctions in the cells ability to generate and process mechanical forces

DNA for each IP sample was eluted with the elution buffer (50 mM Tris-HCl, pH = 8.0, 10 mM EDTA, 1.0% SDS) and reverse cross-linked at 65 C oven for 13.5 h, followed by protease K digestion at 55 C for 2 h