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Description

We also analyzed ChIP-seq datasets of histone H3 modifications, including H3K4me3, H3K4me1, H3K27ac, and H3K27me3, previously generated by us from human HEK293T cells 27

Reverse transcription of total RNA involved using the iScript kit (BioRad)

Hydrogen Water vs

Other details are described in Supplementary Methods

The prevalence of sex-biased AS events in gonadal tissues, marked by 581 multi-isoform genes in testes and 787 in ovaries, underscores AS as a pivotal regulatory mechanism in sex differentiation